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It catalyzes the development in the phosphodiester bond in double-stranded DNA made up of cohesive ends.
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DNA ligase is really a style of enzyme that facilitates the becoming a member of of DNA strands together by catalyzing the formation of a phosphodiester bond. It plays a role in fixing solitary-strand breaks in duplex DNA in residing organisms, but some forms (for example DNA ligase IV) may specially maintenance double-strand breaks (i.
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Quite a few laboratories purified DNA ligases from distinct organisms, which are used in gene cloning to join DNA molecules alongside one another to form recombinant DNA.
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